Table I.

Chlamydia pneumoniae synthetic peptides: protein sources and H-2b binding affinities

IDPeptide SequenceaProtein(s)bcCpn Genome AnnotationsdH-2b MotifeRatio MFIfBinding (IC50 nM)g
1NTVVFDAL60 kDa Omp (Omp2, OmcB)b hCPn0557, CP0195Kb1.69155,000
2QESCYGRLKb2.78344
3ISVSNPGDLDb2.54220
4VLSFNLGDMDb1.4962,857
5AEDTNVSLIDb0.95440,000
6SKLQYKIIFKBP-type PPIASE (Mip)CPn0661, CP0086Kb1.13155,000
7SSEGNNEPILDb/Db1.01293,333
8QLPPNSLLIDb1.07293,333
9DDEEYVIL10 kDa chaperonin (GroES)b hCPn0135, CP0637Kb0.99124,000
10ANEGYDAL60 kDa chaperonin (GroEL)b hCPn0134, CP0638Kb0.9711,923
11TAGANPMDLDb1.4722,000
12ISANNDSEIDb1.80880
13STEINQPFITMDnaK (heat shock protein-70)b hCPn0503, CP0251Db/Kb2.01/2.07124,000/13,209
14VLSTNGDTLDb3.30427
15FLLFFEFLLV76 kDab hCPn0728, CP0018Kb/Kb1.8520,667
16LMSGFRQMKb1.436,889
17YASDNQAILDb2.89480
18GFKSNFNKIKb/Db1.2/0.95155,000/440,000
19LVYNYPGV43 kDa homologs 1–4:4CPn0929, CP0937Kb3.2023
20LIYNYPGV3CPn0928, CP0938Kb2.994.1
21LLVFNYPGI2CPn0927, CP0939Db/Kb1.69/1.485,167/62,857
22LIFNYPGV1CPn0562, CP0188Kb2.8140
23HPYLYRLL1CPn0562, CP0188Kb2.10194
24HPTLFKVL2CPn0927, CP0939Kb2.87620
25SIILFLPL2CPn0927, CP0939Kb3.611.0
26KICQNFILL1CPn0562, CP0188Db/Kb2.80/2.952,480/2,200
27ISNGNSDCL1CPn0562, CP0188Db3.62314
28YSQGNSGLM2CPn0927, CP0939Db2.35889
29TGKLNLENL3CPn0928, CP0938Db1.7917,600
30QAPTNRWML4CPn0929, CP0937Db3.075.6
31SLLGNATALMOMP (OMP1, OmpA)b hCPn0695, CP0051Db2.98160
32SHYAFSPMFEVLOmp5b h (Pmp10)CPn0449, CP0303Kb/Kb2.66689
33ISFAFCQLKb2.911.3
34QPQNYLRLOmp4b.h (Pmp11)CPn0451, CP0302Kb1.5451,667
35HDQLFSLLKb1.892,296
36GTYHFTKLOmp85 homolog (YaeT)CPn0300, CP0458Kb2.7918
37FQLCNSYDLOmpB (PorB)CPn0854, CP1015Db4.2913
38NHPVFSPLIncA homologCPn0585, CP0163Kb2.6156
39LQQRYSRLKb2.910.6
TcTSAVDYNFTIVT. cruzi TSA1Kb3.3736
OVASIINFEKLOVAKb3.3811
FLUnpASNENMETMInfluenza A nucleoproteinDb3.476.2
  • a Sequence in single-letter amino acid code.

  • b Cpn proteins from which H-2b motif-bearing sequences were selected for peptide synthesis; synonyms shown in parentheses.

  • c Originally reported in Cpn genome sequencing Refs. 33–35.

  • d Gene name designations as annotated from the sequenced genomes of Cpn strains CWL029 (prefix CPn; Chlamydia Genome Project; reported in Ref. 33; http://chlamydia-www.berkeley.edu;4231) and AR39 (prefix CP; reported in Ref. 34; http://www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl?database=bcp). Cpn strain J138 gene names are commonly those of CWL029 (http://w3.grt.kyusbu-u.ac.jp/J138/; http://www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl?database=ntcp02).

  • e H-2b binding motif present in synthetic peptides; H-2Kb binding motif is FY at position 5, and LMIV at position 8; H-2Db binding motif is N at position 5, and MIL at position 9 (36).

  • f Binding affinity as assessed by ability of peptides (10 μM) to up-regulate and stabilize Kb or Db molecules on RMA-S cells. Ratio MFI is MFI in the presence of test peptide over the fluorescence intensity in the absence of peptide (high, ≥2.5; intermediate, 2.0–2.4; low, 1.5–1.9; negative, ≤1.4).

  • g Binding affinity as measured by quantitative binding assay. IC50 is the nanomolar concentration of peptide capable of inhibiting by 50% the binding of 125I-labeled index peptide to purified soluble H-2b molecules (high, ≤50 nM; intermediate, 50–500 nM; low, 500–10,000 nM; negative, >10,000).

  • h Originally reported in Refs. 39–44.