Table I.

Oligonucleotide sequences of the primers used in LM-PCR and CHART-PCR assays

Primer SetOligonucleotide SequenceMelting Temperature (°C)
LM-PCR oligonucleotides
A
115′-TGTTTACTCTTGCTCTTGTCCACCAC-3′60
125′-TTGTCCACCACAATATGCTATTCAC-3′60.5
135′-CACATGTTCAGTGTAGTTTTATGAC-3′61
B
565′-CACCTAAGTGTGGGCTAATGTAAC-3′60
575′-AACAAAGAGGGATTTCACCTACATCC-3′61
585′-TTCACCTACATCCATTCAGTCAGTC-3′62
Linker Bottom5′-GAAGAGAAGGT-3′
Linker Top5′-CCAAACGCCATTTCCACCTTCTCTTC-3′
CHART-PCR oligonucleotides
ACoding5′-CGTTAAACAGTACCTCAAGCTCAA-3′
Noncoding5′-CCTTTTTATCCACACAAAGAGCTA-3′
BCoding5′-GGGCTAATGTAACAAAGAGGGATT-3′
Noncoding5′-AACCCATTTTTCCTCTTCTGATGA-3′
CCoding5′-CTACTCACAGTAACCTCAACTCCT-3′
Noncoding5′-TGTAGAACTTGAAGTAGGTGCACT-3′