Table I.

Effect of M. vaccae on IL-4 and IFN-γ release by splenocytes in response to in vitro anti-CD3 mAb and OVA stimulationa

IL-4 (pg/ml)IFN-γ (pg/ml)
SalineM. vaccaeSalineM. vaccae
RPMI2 ± 13 ± 122 ± 1117 ± 6
Anti-CD3667 ± 63b629 ± 93b1346 ± 10b1333 ± 29b
OVA122 ± 30b154 ± 20b28 ± 630 ± 4
  • a Splenocytes from immunized mice treated with either saline or M. vaccae were incubated with RPMI alone or were stimulated either with 4.5 μg/ml immobilized anti-CD3 mAb 145-2C11 or 80 μg/ml OVA. The levels of IL-4 and IFN-γ (picograms per milliliter) in the supernatant of cell cultures were determined by ELISA. Data are the mean ± SEM of six mice for each group.

  • b p < 0.05 compared with saline or M. vaccae-treated and RPMI-stimulated cells.