Table I.

Percentage of reduction on membrane and intracellular protein abundance by extracts of cigarette, loose leaf, and snuff tobaccoa

Cell LinemClass IbTAP1
CigaretteLoose leafSnuffCigaretteLoose leafSnuff
HaCaT504465302933
183565557404033
HeLa575039N.T.bN.T.N.T.
Jesthom636468N.T.3940
Primary keratinocytes424270453946
Average± SDc55 ± 553 ± 761 ± 1438 ± 833 ± 838 ± 6
  • a For each cell population data are expressed as the percentage of reduction of membrane expression of HLA class I or intracellular abundance of TAP1 in tobacco-treated compared to control cells. Data are compiled from multiple experiments. All data were generated by flow cytometry, TAP1 data were generated by intracellular flow cytometry.

  • b mClass I, membrane class I; N.T., Not tested.

  • c For mClass I and TAP1 the overall reduction of each extract on different cell populations was averaged and SDs were generated.