Table II.

Effect of motif position on immunostimulatory activitya

Location of Motifs (5′→3′)No. of Cytokine-Secreting Cells
IL-6IL-12IFN-γ
CpG ODNb79 ± 31980 ± 230260 ± 40
[CpG-Sup] ODNb72 ± 142080 ± 480230 ± 60
[Sup-CpG] ODN0 ± 0140 ± 300 ± 0
[CpG-Cont] ODNb64 ± 122210 ± 130284 ± 34
[Cont-CpG] ODNb80 ± 111942 ± 88238 ± 28
[Cont-Sup] ODN8 ± 2184 ± 3436 ± 8
[CpG-Sup] ODN+ Sup ODN4 ± 2226 ± 3828 ± 6
[Sup-CpG] ODN+ CpG ODN7 ± 3250 ± 3234 ± 9
  • a BALB/c spleen cells (106) were coincubated with 1 μM of each ODN. Complex ODN (20 bp in length) were constructed from 10-mer encoding suppressive (Sup; GAGCAAGCTG and AGCTTGAGGG), stimulatory (CpG; TCACGTTGA and TAGACGTTAG), or control (Cont; TCAAGCTTGA and TAGAGCTTAG) motifs. The number of cytokine-secreting cells per 106 cells was determined by ELISPOT after 24 h of stimulation. Results represent the average ± SD of triplicate assays involving at least two ODN of each type.

  • b Stimulatory ODN, p < 0.05.