Table I.

Effect of mixing cells treated with suppressive vs stimulatory ODNa

Suppressive ODNODN Added During Culture% Maximal Cytokine Production
PretreatmentFresh CellsIL-6IL-12
+CpG100 ± 13100 ± 6
+Control3 ± 27 ± 2
+CpG9 ± 66 ± 2
+Control0 ± 00 ± 0
++CpG86 ± 16105 ± 12
++Control0 ± 00 ± 0
  • a BALB/c spleen cells were treated with 1 μM suppressive ODNH154 for 2 h and then washed (first column). These cells were added to naive splenocytes (second column) plus 1 μM control (ODN1471) or CpG (ODN1555) ODN. IL-6 and IL-12 levels in culture supernatants were measured by ELISA after 18 h. The percentage of maximal cytokine production was calculated by the formula: (cytokine produced by treatment group) − (background)/(cytokine produced by fresh cells stimulated with CpG ODN) − (background) × 100%, where the background was cytokine levels in fresh cells cultured in medium alone. Results represent the average ± SD of triplicate assays, each standardized to the response induced by bacterial DNA (62 pg/ml IL-6; 134 pg/ml IL-12).