Table IV.

Clonal deletion in IL-10−/− C57BL/6 hosts conditioned with ATS, TLI, and given BALB/c heart and bone marrow transplants

Host TypeVβ2aVβ3V1024Vβ5.1/5.2Vβ6Vβ9Vβ11Vβ12
BALB/cWild typeControl0.26 ± 0.010.02 ± 0.010.30 ± 0.040.03 ± 0.000.40 ± 0.090.10 ± 0.000.05 ± 0.000.03 ± 0.02
C57BL/6Wild typeControl0.26 ± 0.030.17 ± 0.060.29 ± 0.030.35 ± 0.040.40 ± 0.120.13 ± 0.020.36 ± 0.040.16 ± 0.04
C57BL/6IL-10−/−Chimerab0.25 ± 0.04 0.03 ± 0.01 0.47 ± 0.02 0.07 ± 0.03 0.44 ± 0.020.11 ± 0.01 0.09 ± 0.04 0.05 ± 0.02
C57BL/6Wild typeChimerab0.24 ± 0.02 0.02 ± 0.01 0.40 ± 0.05 0.06 ± 0.04 .28 ± 0.070.06 ± 0.01 0.07 ± 0.02 0.03 ± 0.02
  • a The ratio equals the percentage of Vβn+ to the percentage of Vβ8+ T cells. Mean percentage of Vβ+ T cells of BALB/c wild-type control was 21.6 ±1.3%. Mean percentage of Vβ8+ T cells amongst H-2Kb+ T cells of C57BL/6 wild-type control, IL-10−/−, and wild-type chimeras was 16.3 ±1.1, 21.9 ±1.2, and 20.8 ±4.2%, respectively. Bold shows Vβ ratios indicative of clonal deletion. Mean and SE of three replicate experiments are shown.

  • b Ratio amongst gated H-2Kb+ CD3+ host-type cells.