Table I.

Characteristics of primer sets used to detect TLRs and IL-8

Target GeneAccession CodeaAmplicon PositionAmplification EfficiencyGenomic DNA (5 ng/ml)Transcripts/2 × 106 PBMC (n = 3)HEK293 300 cells
Cyclophilin-BM60857105–3581.95n/ab1.6 × 10612,396
IL-8XM_003501101–3661.90n/aNDND
TLR1NM_0032631919–22541.86886415,7041
TLR2NM_0032641549–18191.90864323,9710
TLR3NM_0032651505–17171.9385816,13412
TLR4NM_003266439–7671.911130317,5300
TLR5U88881733–9821.9369537,75722
TLR6NM_0032682378–26501.931791117,2694
TLR7AF245702774–10121.9856323,9720
TLR8AF2457032296–25901.9583160,7900
TLR9AF2457042741–29411.9585366,7992
TLR10AF2966731767–20541.9078553,2670
  • a GenBank, National Center for Biotechnology Information.

  • b n/a, Not applicable, because primers were designed to be intron over-spanning.