Table I.

Effects of BCD on mAb binding

mAbControl MFIa (% pos)BCD MFIb (% pos)
Mouse IgG2a2.95 (2.1)3.20 (4.8)
Anti-CD4407.5 (100)412.9 (99.9)
Anti-CD451440 (100)1571 (100)
12G5309.8 (99.3)191.5 (99.4)
  • a MFI of the entire gated population; % pos, percent positive above 98% of the IgG2a background.

  • b Cells were treated with 20 mM BCD in PBS as described in Materials and Methods.