Table III.

VH and VL usage in mAbs derived from MAP-DWEYSVWLSN-immunized B/W micea

dsDNA/PeptideVHDHJHVkJk
B/W1,19-2+/+J558DFL16.12VOx-14
B/W1,17-15+/−J558DQ523Vk1-b1
B/W2,18-2−/−J558?2Vk212
B/W2,27-7+/−J558DFL16.2c3Vk1-a4
B/W1,23-37+/+VH10?3Vk212
B/W2,25-32+/+Q52DFL16.22Vk82
B/W3,12-5+/+J558DQ522Vk81
B/W2,28-22+/−J558DFL16.12Vk1-a4
B/W2,7-13+/+J558DFL16.1c2Vk91
B/W3,14-1+/+J558DFL16.11Vk1-c5
B/W1,21-28+/+Q52DSP2.34Vk211
B/W2,24-13+/+Q52DFL16.22Vk82
B/W3,11-6+/+J558DQ522Vk81
B/W3,24-4+/+J558DFL16.12Vk211
B/W2,19-19+/+J558DSP2.64Vk41
B/W2,3-4+/−J558DQ522Vk1-c2
B/W2,30-4−/−J558DFL16.23Vk82
B/W3,29-2+/+J558DFL16.13Vk1-c1
B/W3,20-3+/+J558DSP2.94Vk42
B/W3,9-18+/+J558DSP2.63Vk324
B/W2,30-2+/−Q52DFL16.24Vk81
  • a Total RNA from 2 × 107 hybridoma cells was reversed transcribed with an oligo(dT) primer. PCR for the H and L chain genes was performed using gene-specific primers, and V region sequences were compared to murine Ig sequences in GenBank using the Advanced BLAST program from the National Center for Biotechnology Information. The column labeled dsDNA/Peptide details mAb specificity for dsDNA and/or peptide.