Table I.

Antigenic specificities of mAbs isolated from MAP-DWEYSVWLSN-immunized B/W micea

IsotypedsDNAssDNAPeptideCardiolipinHistoneSm/RNP
B/W1,19-2IgM3.53.53.53.52.863.5
B/W1,17-15IgM 3.5 3.5 0.170.160.120.14
B/W2,18-2IgM0.10.120.25 3.5 0.130.17
B/W2,27-7IgM 3.5 1.49 0.09 0.43 0.16 0.78
B/W1,23-37IgM0.410.41.512.460.4 0.71
B/W2,25-32IgM2.042.652.963.10.611.08
B/W3,12-5IgM3.53.51.452.010.140.24
B/W2,28-22IgM 3.5 2.48 0.15 1.08 0.14 0.91
B/W2,7-13IgM 3.24 2.86 0.29 0.240.32 0.57
B/W3,14-1IgM3.53.53.53.50.921.67
B/W1,21-28IgM0.440.871.330.771.141.86
B/W2,24-13IgM 1.45 2.39 2.48 2.35 0.49 0.74
B/W3,11-6IgM 3.5 3.5 1.08 1.06 0.150.22
B/W3,24-4IgM 0.67 1.52 0.68 1.56 0.120.16
B/W2,19-19IgG10.310.761.661.580.750.77
B/W2,3-4IgG2a 3.5 3.5 0.120.120.140.15
B/W2,30-4IgG2a0.10.160.22 1.54 0.140.13
B/W3,29-2IgG2b0.361.02.041.791.050.89
B/W3,20-3IgG2b3.52.71.051.523.51.8
B/W3,9-18IgG2b 0.24 0.42 1.58 0.93 0.46 0.83
B/W2,30-2IgG3 0.41 0.63 0.76 0.56 0.31 0.55
  • a Normalized supernatants at 10 μg/ml were incubated on Ag-coated plates for 2 h, followed by the appropriate alkaline phosphatase-linked goat anti-mouse Ig and substrate. Numbers reflect OD readings at 405 nm. mAbs are defined as positive (in bold) for a given specificity if the OD value was greater than the average of at least two isotype-matched control Abs plus 2 SD.