Table I.

Cytotoxicity and cytokine production by CTL on coincubation with various cell linesa

HistologyH-2dbmAbLysis (%)IFN-γ (pg/ml)TNF-α (pg/ml)
RencaRenal cell cancer+54>8,000488
Anti-CD457>8,000441
Anti-CD81200
Anti-H2-Kd1693197
Anti-H2-Dd45>8,000527
STRCC-1Renal cell cancer+0
P815Mastocytoma+400
C26Colon cancer+000
MethAFibrosarcoma+13
L10ALymphoma+0130
A20Leukemia+2150
Harvey BALBFibroblast000
Fibroblast+510
ConA splenic blast+0
B16Melanoma200
L929Fibrosarcoma34
  • a Cytotoxicity against various lines was measured in a 16 h 111In-release assay at an E:T ratio of 20. CTL and stimulator cells were cultured for 16 h (E/S = 4), and IFN-γ and TNF-α release of supernatant was measured by ELISA. For the inhibition experiments, blocking mAb was added to the culture at the concentration of 10 μg/ml. The representative data of three individual experiments are shown.

  • b Expression of H-Kd molecule on cell surface was tested by flow cytometer analysis.