Table I.

Oligonucleotides used for PCR from bisulphite-treated genomic DNAa

DetailsSequencesPosition
GenBank J00219
hifng.F3(nc) IFNγ fwd5′-TATAAATAAAAAATCAACATTTTACCAAAA21–50
hifng.R3a(nc) IFNγ rvse5′-TTGGTAGTAATAGTTAAGAGAATTTA546–521
hifng.F3a(c) IFNγ fwd5′-TGTGAATGAAGAGTTAATATTTTATTA21–47
hifng.R2(c) IFNγ rvse5′-TTCTACTTCTTTTACATATAAATCCTAACA541–570
GenBank M23442
hil4.F3(nc) IL-4 fwd5′-CCTCTATACAAAAAAAAAACCCCAAAT621–647
hil4.R3a(nc) IL-4 rvse5′-TATGTTAGTAGGAAGAATAGAGGGG1,196–1,220
hil4.F2a(c) IL-4 fwd5′-TGTTATTTTGTTTTTATGTAGAGAAGGA611–638
hil4.R2(c) IL-4 rvse5′-ATTACCAACACATACTAACAAAAAAAACAA1,201–1,230
GenBank X02910
TNF.F3(nc) TNFα fwd5′-ACCCAACCTTTCCTAAAACCTCAAA51–75
TNF.R1a(nc) TNFα rvse5′-GTTGTTTTTAGGGGGGGTTTGTAG675–698
GenBank AL050337(rvse complement)
ifngr.F1(nc) IFNGRI fwd5′-AAATTCCTCAAATAAAAAAACAAAAACT32,401–32,428
ifngr.R2a(nc) IFNGRI rvse5′-YGYGGTGTTTATTTTAGTTTTGTTTAT32,914–32,940
ifngr.F1a(c) IFNGRI fwd5′-AGGTTTTTTAAATGAAAAAGTAGGGAT32,401–32,427
ifngr.R2(c) IFNGRI rvse5′-CRCRATACCCATCTCAACCCTA32,919–32,940
  • a Oligonucleotide details include whether the primer was designed against bisulphite-treated coding (c) or noncoding (nc) strand. fwd, forward; rvse, reverse.