Table III.

NO production by BM-DCa

NO2 (μM)
PGE2LPS (1 μg/ml)LPS (1 μg/ml) + NS-398LPS (1 μg/ml) + l-NMMA
BM-DC1.04 ± 0.121.50 ± 0.2058.10 ± 02b55.50 ± 035.65 ± 0.6b
T cells0.80 ± 0.100.60 ± 0.200.500 ± 0.10.7000 ± 0.40.90 ± 0.30
  • a A total of 106 BM-DC/ml or an equivalent number of T cells were incubated for 48 h in the presence of 1 μg/ml LPS or 1 μM PGE2. Parallel cultures were run with NS-398 (10 μM) or l-NMMA (0.5 mM). Supernatants were collected and analyzed for NO2 release. Results are the mean ± SD obtained from triplicate cultures and are representative of six separate experiments.

  • b Values of p < 0.01.