Table II.

Selective COX inhibitors dose-dependently inhibit PGE2 production from BM-DC in the absence of exogenous AAa

SC-560 (nM)BM-DC Treated with LPS (1 μg/ml)
SC-560 (nM)NS-398 (μM)
010204001020400102040
PGE2819 ± 65450 ± 4741 ± 925 ± 43670 ± 1543266 ± 1912973 ± 1522936 ± 1873670 ± 191697 ± 56624 ± 59588 ± 43
(pg/ml)(100)(55)(5)(3)(100)(89)(81)(80)(100)(19)(17)(16)
  • a Cells matured in the presence of LPS (1 μg/ml) were treated with graded concentrations of selective COX-2 inhibitor, NS-398, or selective COX-1 inhibitor, SC-560 for 48 h. PGE2 production (expressed in picograms per milliliter per 106 cells) was measured by ELISA, and the percentage of PGE2 production was calculated. The effect of COX-1 selective inhibitor was also tested in the absence of LPS stimulation. Results are the mean ± SEM of four independent experiments.