Table I.

Eicosanoid production by BM-DCa

Unstimulated CellsBM-DC Treated with LPS (1 μg/ml)
NoneIndo (4 μM)SC-560 (20 nM)NS-398 (10 μM)Indo + NSSC + NSNDGA (1 μM)
PGE2819 ± 653670 ± 154b2833 ± 1122973 ± 152697 ± 56b256 ± 31b198 ± 24b3679 ± 171
PGE2 (+ AA)906 ± 623790 ± 152b2850 ± 1732956 ± 123755 ± 95b265 ± 20b260 ± 30b3755 ± 167
LTB4680 ± 561224 ± 1121380 ± 0881466 ± 1352033 ± 1472433 ± 1752451 ± 177204 ± 27b
LTB4 (+ AA)702 ± 321240 ± 1021450 ± 1251500 ± 1402145 ± 1542450 ± 1952650 ± 187248 ± 21b
  • a Formation of PGE2 and LTB4 was measured by ELISA in supernatants from control cells and LPS-stimulated cells, with and without addition of 1 μM exogenous AA. Some LPS-stimulated BM-DC were treated with COX inhibitors (4 μM indomethacin, 20 nM SC-560, 10 μM NS-398) or 5-LO inhibitor (NDGA at 1 μM). Controls included BM-DC stimulated with LPS (1 μg/ml) and BM-DC cultured alone. Results (expressed in picograms per milliliter per 1 × 106 cells) represent the mean ± SEM of six different experiments.

  • b Values of p < 0.01 by Student’s t test when compared to controls.