Table I.

Clonogenic capacities of TCR-transgenic CD8 T cell populationsa

MiceNaivePrimedbTwice Primedc
F5379 ± 232 (n = 3)14.8 ± 8.6 (n = 2)9 ± 1.1 (n = 6)
Rag−/−F561 ± 46 (n = 4)9.9 ± 0.7 (n = 2)4.2 ± 1.3 (n = 3)
  • a Serial dilutions of splenocytes from the indicated mice were cultured in the presence of IL-2, 10 nM NP68 peptide, and irradiated syngeneic splenocytes. The frequency of clonable cells was calculated as described in Materials and Methods. The results are expressed as the inverse of the frequency of clonable cells (mean ± SD of n independent experiments).

  • b Primed mice were immunized i.p. with 50 nmol NP68 peptide.

  • c Twice-primed mice were immunized i.p. twice at a 24-h interval with 50 nmol NP68 peptide.