Table I.

Recognition of SSX-2-derived peptides by LAU 50 TILNa

PeptideSequenceScoreNo. of Spots
SSX-25–13DAFARRPTV175
SSX-27–15FARRPTVGA155
SSX-215–24AQIPEKIQKA163
SSX-216–24QIPEKIQKA214
SSX-240–49MKASEKIFYV1639
SSX-241–49KASEKIFYV2269
SSX-250–59YMKRKYEAMT153
SSX-253–61RKYEAMTKL154
SSX-257–65AMTKLGFKA164
SSX-258–67MTKLGFKATL177
SSX-259–67TKLGFKATL1912
SSX-2103–111RLQGISPKI236
  • a HLA-A2-binding peptides (as predicted by motif-based algorithm) for which the C terminus could be generated in vitro by proteasome digestion of 22-aa precursors (Fig. 1). Theoretical binding score given by motif-based prediction algorithm in SYFPEITHI database (http://www.syfpeithi.de; Ref. 20 ). Number of spots obtained by IFN-γ ELISPOT of LAU 50 TILN. Short term-cultured TILN from patient LAU 50 (2 × 104 cells/well) were tested by IFN-γ ELISPOT in the presence of T2 cells (5 × 104 cells/well) alone or in the presence of the indicated peptide in duplicates. The number of spots obtained in the absence of peptide or in the presence of control peptide HIV-RT476–484 was 3 and 13, respectively.