Cytokine production by lung myeloid DC upon exposure to conidia or hyphae of A. fumigatus
| Exposure toa | Cytokine Productionb | |||
|---|---|---|---|---|
| TNF-α | IL-12p70 | IL-4 | IL-10 | |
| None | <32 | <16 | <4 | <0.6 |
| Conidia | 65 ± 12c | 41 ± 8c | <4 | <0.6 |
| Hyphae | 117 ± 8c | <16 | 18 ± 2c | 8 ± 3c |
a Myeloid DC were isolated from lungs of BALB/c mice and exposed to viable conidia or hyphae (at cells, fungi ratios of 1:5 and 1:1, respectively) for 24 h before determination of cytokines in culture supernatants. Amphotericin B was added to the cultures for the last 22 h to prevent fungal overgrowth.
b Levels of cytokines (pg/ml; mean ± SE), as determined by specific ELISA; <, below the detection limit of the assay.
c p < 0.05, pulsed vs unpulsed DC.