Table II.

Cord blood lymphocyte cytokine responses to MSP-1 P2 and P3 peptidesa

Subject NumberCell PreparationIFN-γ (pg/ml)bIL-13 (pg/ml)b
Media aloneP2 peptideP3 peptideMedia aloneP2 peptideP3 peptide
1CBL000000
2CBL000000
3CBL000000
4cCBL10127023339193124
CD4 depletedd000000
5cCBL0000479122
CD4 depleted000000
6cCBL000000
CD4 enriched000000
7cCBL000000
CD4 enriched0265268000
8CBL62288327000
9CBL000000
CD4 enriched0138127000
CD8 enriched000000
Non-CD4/CD8000000
10CBL000000
11CBL000000
12CBL000000
  • a MSP-1 P2 and P3 peptides correspond to amino acids specified by codons 250–271 and 1101–1121 (GenBank accession number AF06234).

  • b IFN-γ and IL-13 production following a 72-h incubation period were determined by ELISA as described in Materials and Methods.

  • c Cord blood from these subjects was positive for P. falciparum small subunit rDNA by PCR.

  • d CD4 and CD8 cell depletion were performed by immunomagnetic bead selection as described in Materials and Methods.