Table I.

Surface expression of MAC-1 on murine microglial cellsa

Percentage of positive cells
N9PrimaryRAW264.7
LPS−b968686
LPS+94NTc91
  • a N9 cells and primary murine microglial cells were incubated with FITC-conjugated rabbit anti-murine MAC-1 Ab for 30 min on ice. The cells were then washed and examined for fluorescence by FACS analysis. A murine macrophage cell line, RAW264.7, was used for comparison.

  • b Cells were incubated in the absence (−) or presence (+) of LPS for 24 h at 37°C, before staining with anti-MAC-1.

  • c NT, not tested.