Table III.

Localization of the epitope on NS3 recognized by CTL isolated from subject C94-094a

Expt.Target Cells% Specific Lysis by Indicated T Cell Line
2D62D102D51D6
1vvD3:NS3/1–61848403051
vvD3:NS3/1–35463354454
vvD3:NS3/1–24761524163
vvD3:NS3/1–2161011
vvD3:NS3/1–8300
vvControl1000
2D4-NS3221–235ND596077
D4-NS3221–23277494980
D4-NS3221–2290020
D4-NS3222–2300111
D4-NS3224–2351210
No peptide1023
  • a The E:T cell ratios were between 10:1 and 20:1. —, Not tested. In experiment 1, autologous BLCL were infected with recombinant vaccinia viruses (vv) expressing different truncations of the NS3 protein. In experiment 2, autologous BLCL were pulsed with 25 μg of the indicated peptide. Boldface values indicate significant target cell lysis.