Table I.

LXA4 analog attenuates activation of proinflammatory gene expressiona

Gene NameAccession No.Control Fold InductionLX Fold InductionLX/Control Induction Ratio
Small inducible cytokine subfamily A (Cys-Cys), member 20D8695531.814.20.45
TNF-α-induced protein 3M5946523.417.90.75
Baculoviral IAP repeat-containing 3AI58149922.59.90.44
Superoxide dismutase 2, mitochondrialY00472116.40.58
Nuclear factor of κ light gene enhancer in B-cells inhibitorM6904310.97.80.72
Apoptosis inhibitor 2 (baculoviral IAP repeat-contining 3)U375469.63.90.41
Leukemia inhibitory factor (cholinergic differentiation factor)X139678.85.90.67
Matrix metalloproteinase 7 (matrilysin, uterine)L225247.13.10.44
Heat shock 70-kDa protein 1AM598286.30.590.09
ESTs (chromosome 8 open reading frame 4)AA1283056.37.81.24
Syndecan binding protein (syntenin)AF00065262.30.38
ESTs (hypothetical protein FLJ23231)AI22523565.10.85
Apoptosis inhibitor 1 (baculoviral IAP repeat-contining 2)U375475.93.30.56
UDP-Gal:βGlcNac β 1,4-galactosyltransferase, polypeptide 1X132235.82.10.36
Heat shock 70-kDa protein 8 (HSC71)AL0441725.30.670.13
c-fos oncogeneV015125.22.90.56
Protease inhibitor 3, skin-derived (SKALP)D13156520.4
Platelet factor 4 variant 1M261674.93.70.76
GTP-binding protein overexpressed in skeletal muscleU105504.72.50.53
Phosphoprotein regulated by mitogenic pathwaysAJ0004804.23.60.86
Heat shock 70-kDa protein (HASP70B′)X517573.90.770.2
Heat shock 105-kDaAB0033343.80.590.15
DnaJ (Hsp40) homolog, subfamily B, member 4U409923.80.710.19
Transmembrane protease, serine 2U753293.830.79
Prostate differentiation factorAA2166853.83.40.89
NK cell transcript 4AI5390553.72.50.68
Proplatelet basic proteinM549953.72.90.78
Tissue inhibitor of metalloproteinase 3AI2454713.730.81
IFN (α, β, ω) receptor 2L419423.73.10.84
Jagged1 (Alagille syndrome)U612763.61.70.47
Cytokeratin 20X735013.41.50.44
Cyclin-dependent kinase inhibitor 1A (p21, Cip1)L256103.42.10.62
E74-like factor 3 (ets domain transcription factor)U668943.43.20.94
ESTs (possessing ankyrin repeats)AI7444783.330.91
ESTs (dual adapter of phosphotyrosine and 3-phosphoinositides)AA1498683.22.20.69
Nuclear receptor subfamily 4, group A, member 1L137403.22.30.72
Down syndrome candidate region 1U852673.23.61.13
Ring finger proteinY078283.11.60.52
Human Bcl-2 binding component 3 (bbc3)U829873.12.60.84
Cytochrome P450, subfamily I (aromat. Comp.-inducible), polypeptide 1K031913.13.21.03
Diphtheria toxin receptorAC00463432.40.8
IFN regulatory factor 1X1445432.90.97
Early growth response 1M805832.91.90.66
Low density lipoprotein receptor (familial hypercholesterolemia)L003522.92.10.72
Small inducible cytokine subfamily B (Cys-X-Cys), member 10X025302.81.50.54
Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein)M867522.70.590.22
Laminin, β 3 (nicein (125 kDa), kalinin (140 kDa), BM600 (125 kDa))U177602.71.70.63
Ubiquitin CAI5651172.72.20.81
KIAA0127 gene productD509172.72.20.81
Nef-associated factor 1AJ0118952.72.50.93
Heat shock 60-kDa protein 1 (chaperonin)M346642.60.710.27
KIAA0291 protein (cytoplasmic linker 2) {GenBank AB006629}AB0066292.61.30.5
Myeloid cell leukemia sequence 1 (BCL2-related)AF1181242.61.50.58
Chromodomain helicase DNA binding protein 4X866912.61.60.62
Growth arrest and DNA-damage-inducible, αAI6346582.62.20.85
CDP-diacylglycerol synthase (phosphatidate cytidylytransferase) 1AI6362232.51.70.68
TNFR superfamily, member 10bAF0162662.520.8
Heat shock 90-kDa protein 1, βM166602.40.830.35
KIAA0585 protein (phosphotidylserine receptor)AB0111572.410.42
(Table continues)
  • a Model epithelia were treated with vehicle (0.1% ethanol or 100 nM 15-(R/S)-methyl-LXA4) for 1 h, and then were colonized for 4 h with S. typhimurium at which time mRNA was isolated and analyzed via cDNA microarray hybridization. GenBank name and corresponding accession number are shown. Control fold induction and LXA4 analog induction are the fold up-regulation for each gene observed in response to S. typhimurium relative to a common control (untreated T84 cell mRNA) in the presence (LX induction) and absence (control induction) of LXA4 analog. LX/control induction ratio is the ratio of control induction to that observed in the presence of LXA4 analog. Data are in descending order of control induction beginning with the most up-regulated genes. NF-κB-regulated genes are shown in bold. Genes for heat shock proteins are italicized.