Table IV.

Summary of the CDR3α sequences of TCRs of position 4 variant-specific CTLs elicited from Tgβ micea

PeptideRecognition of VSV8CDR3 Sequence 93CDR3 LengthJα UsageFrequency
K4No2 RGSALGRLH 91813 /13
No4EXSGSWQLI92221 /26
R4No2EXSSGSWQLI102221 /28
Y4No15LSNYNVLY82131 /38
A4Yes15SXYQGGRALI10158 /10
I4Yes5SXXQGGRALI101513 /19
E4Yes5TXXGGRALI91513 /15
D4Yes1LXQGGRALI91516 /25
  • a Mice were immunized with the indicated peptides, and CDR3α sequences were determined after 2 wk of culture in the presence of the immunizing peptide. The single-letter amino acid code is used. CDR3α is taken to be from position 93 to just before the Jα-encoded “FG.” Conserved CDR3 residues are shown in boldface, and the Jα region is underlined. X, Nonconserved residues. The CDR3 length indicated is for the listed sequence only. “Recognition of VSV8” indicates whether the position 4 variant-specific CTLs can cross-react with VSV8. Frequency, Number of each listed amino acid sequence obtained relative to the total number of sequences analyzed for that family.