Table II.

CDR3α sequences of N30.7 and VSV8-specific CTLs of the Vα2 family from a representative Tgβ mousea

T cellsCDR3 Sequence 93CDR3 LengthJα UsageFrequency
N30.72PAYQGGRALI1015
Tgβ CTLs2PAYQGGRALI10151 /12
PYQGGRALI9152 /12
PSVYQGGRALI11151 /12
PHYQGGRALI10152 /12
PTYQGGRALI10152 /12
PGQGGRALI9151 /12
  • a Tgβ mice were immunized with VSV8, and TCR CDR3α sequences were determined after 2 wk of culture in the presence of VSV8. The single-letter amino acid code is used. CDR3α is taken to be from position 93 to just before the Jα-encoded “FG” (with the conserved C taken to be at position 90). Conserved CDR3 residues are shown in boldface and the Jα region is underlined. Frequency, Number of each listed amino acid sequence obtained relative to the total number of sequences analyzed for that family. The N30.7 sequence was previously reported (24 ).