Table I.

Peptides used for immunization of Tgα and/or Tgβ micea

PeptideNotationPeptide Sequence
Wild typeVSV8R G Y V Y Q G L
Position 1 variantsK1K – – – – – – –
E1E – – – – – – –
I1I – – – – – – –
Position 4 variantsA4– – – A – – – –
I4– – – I – – – –
D4– – – D – – – –
E4– – – E – – – –
K4– – – K – – – –
R4– – – R – – – –
Y4– – – Y – – – –
Position 6 variantsK6– – – – – K – –
R6– – – – – R – –
D6– – – – – D – –
E6– – – – – E – –
  • a Positions 1, 4, and 6 of VSV8 are the potential TCR contacting residues (18, 26) and are underlined and bolded. These residues were substituted in the peptide variants as indicated, and the notation for each variant indicates the substituting residue and its position. In the variant peptide sequences, a dash indicates that the same residue as in VSV8 is present at that position. In general, single amino acid changes in the TCR contacting residues of VSV8 do not dramatically alter the affinity of the peptide for H-2Kb (Refs. 27 ,28 ,29 , and data not shown) or its conformation within the peptide-binding groove (28, 30).