Table II.

Cytokine depletion from NKT-S

NDepleted Cytokine(s)Cytokine Concentration (pg/ml)a
IL-2IFN-γTNF-α
1NKT-S11641128417260
2IL-2<201170217079
3IFN-γ12422518526
4TNF-α121611075<20
5IL-2+ IFN-γ<204017405
6IL-2+ TNF-α<2010875<20
7IFN-γ+ TNF-α108525<20
8IL-2+ IFN-γ+ TNF-α<2040<20
  • a Cytokines were detected by CBA analysis in the cell-free supernatant collected from hCD1d/αGalCer stimulated NKT cells before and after depletion of the indicated cytokines using neutralizing Abs and protein-A Sepharose 4 Fast Flow medium.