Table VI.

NK1.1 vs CD1d/α-GalCer tetramer labeling of T cells between strainsa

OrganStrain% αβTCR+ Cells That Express NK1.1% αβTCR+ Cells That Bind CD1d/α-GC% NK1.1+ T Cells That Do Not Bind CD1d/α-GC% CD1d/α-GC+ T Cells That Do Not Express NK1.1
ThymusbC57BL/63.6 ± 0.8c4.1 ± 0.811 ± 421 ± 5
NOD.NK1.10.8 ± 0.2*1.0 ± 0.2*30 ± 10*45 ± 8*
BALB.NK1.12 ± 15 ± 218 ± 370 ± 10*
SpleenC57BL/65.6 ± 0.95 ± 134 ± 324 ± 5
NOD.NK1.12.4 ± 0.9*2.4 ± 0.9*70 ± 10*65 ± 6*
BALB.NK1.12.6 ± 0.7*6 ± 141 ± 675 ± 5*
Bone marrowC57BL/624 ± 913 ± 457 ± 218 ± 4
NOD.NK1.19 ± 2*4 ± 178 ± 446 ± 2
BALB.NK1.118 ± 621 ± 1050 ± 1051 ± 4*
LiverC57BL/655 ± 752 ± 612 ± 16 ± 2
NOD.NK1.142 ± 936 ± 8*28 ± 8*17 ± 7*
BALB.NK1.132 ± 9*49 ± 710 ± 240 ± 10*
  • a Cells were labeled with mAb specific for αβTCR, NK1.1, and CD1d/α-GalCer tetramer. αβTCR+ cells were electronically gated and analyzed for their expression of NK1.1 and CD1d/α-GalCer tetramer, as shown in Fig. 3B.

  • b αβTCRhigh cells were analyzed in the thymus.

  • c Results represent mean ± SD for nine mice of each strain, from five individual experiments, except for the bone marrow data, which are derived from five mice from three experiments. CD1d/α-GC, CD1d/α-GalCer tetramer; *, Significantly different from C57BL/6 (p ≤ 0.05); †, significant difference obtained between NOD.NK1.1 and BALB.NK1.1 strains (p ≤ 0.05).