Table I.

Tissue distribution of NK1.1+ T cellsa

OrganStrainnbTotal Cell No. (×10−6)% NK1.1+αβTCR+ (NK1.1+T)% NK1.1+αβTCR (NK)% NK1.1αβTCR+ (NK1.1 T)% T Cells That Express NK1.1
ThymuscC57BL/631200 ± 50d0.45 ± 0.150.05 ± 0.02
NOD.NK1.117145 ± 400.16 ± 0.07*0.10 ± 0.03*
BALB.NK1.125230 ± 900.3 ± 0.1*0.04 ± 0.02
SpleenC57BL/632110 ± 201.2 ± 0.52.5 ± 0.724 ± 35 ± 2
NOD.NK1.12090 ± 250.4 ± 0.1*2.2 ± 0.536 ± 5*1.1 ± 0.2*
BALB.NK1.125130 ± 300.5 ± 0.1*3.9 ± 0.7*27 ± 4*1.7 ± 0.4*
Bone marrowC57BL/62415 ± 40.6 ± 0.21.1 ± 0.42 ± 123 ± 6
NOD.NK1.11012 ± 30.3 ± 0.1*3.2 ± 0.6*4 ± 2*8 ± 4*
BALB.NK1.11912 ± 30.11 ± 0.05*1.0 ± 0.41.0 ± 0.6*11 ± 4*
LiverC57BL/6203 ± 120 ± 69 ± 327 ± 640 ± 10
NOD.NK1.1101.1 ± 0.516 ± 3*14 ± 3*35 ± 6*32 ± 6*
BALB.NK1.1214 ± 211 ± 3*15 ± 3*38 ± 6*22 ± 5*
PLNC57BL/6526 ± 30.39 ± 0.050.8 ± 0.152 ± 20.74 ± 0.08
NOD.NK1.1526 ± 30.13 ± 0.02*0.39 ± 0.09*74 ± 2*0.18 ± 0.03*
BALB.NK1.1531 ± 50.13 ± 0.01*0.57 ± 0.0970 ± 10.18 ± 0.02*
  • a Cells were labeled with mAb specific for αβTCR and NK1.1. Populations were defined based on the quadrants shown in Fig. 1. *, Significantly different from C57BL/6 (p ≤ 0.05); †, significant difference obtained between NOD.NK1.1 and BALB.NK1.1 strains (p ≤ 0.05).

  • b n, No. of samples tested.

  • c The percentage of NK1.1αβTCR+ cells was not determined as most thymocytes are immature T cells.

  • d Results represent mean ± SD for the number of samples indicated, from a minimum of three individual experiments.