Table I.

Oligonucleotide primer for the generation of recombinant immunoreceptorsa

NameSequence
1-CD28-BamHI-sense5′-CTACTCGAGGATCAGGAGCGATAGGCTGCGAA-3′
2-CD28-XhoI-antisense5′-CTGGATCCCTACGTAATGCTCAGGCTGCTCTTGGCT-3′
3-CD28-CD3ζ-antisense5′-GTCTGCGCTCCTGCTGAACTTCACTCTCAGGGAGCGATAGGCTGCGAAGTC-3′
4-CD28-CD3ζ-sense5′-CCTATCGCTCCCTGAGAGTGAAGTTCAGCAGG-AGC-3′
5-CD3ζ-XhoI-antisense5′-CTACTCGAGGATTAGCGAGGGGGCAGGGC-3′
6-Lκ-NcoI-sense5′-CTACGTACCATGGATTTTCAGGTGCAGATTTTC-3′
7-hIgG1Fc-BglII-antisense5′-CCCACCCAGATCTTTTTTACCCAGAGACAGGGAGAG-GCTCTTCTG-3′
  • a Restriction sites are underlined. CD28 and CD3ζ overlapping sequences are in bold face.