Table I.

Comparative analyses of viable cell recovery with the cytokines on blood DC subsetsa

No CytokineIL-3GM-CSFIFN-αIFN-γTNF-αTGF-β1IL-6
CD11c+ DCs36.5 ± 3.451.0 ± 3.3*79.5 ± 3.7**88.3 ± 2.8**83.8 ± 3.0**37.5 ± 2.834.5 ± 2.538.8 ± 3.5
CD11c DCs19.5 ± 2.775.5 ± 3.3**45.3 ± 3.2*73.8 ± 3.1**30.0 ± 2.7*19.5 ± 2.318.8 ± 2.520.8 ± 2.5
  • a After 24-h culture with a panel of cytokines in the serum-free condition, the recovery of viable cells of each DC subset was counted using the trypan blue dye exclusion test. Freshly isolated cells were cultured in 96-well flat-bottom tissue culture plates at 4 × 104 cells in 200 μl of medium/well. Results are shown as percent recovery of viable cells (means ± SEM) of four separate experiments using DCs from healthy individual volunteers. Statistical significance using paired Student’s t test (∗, p < 0.05 and ∗∗, p < 0.001) is indicated.