Comparative analyses of viable cell recovery with the cytokines on blood DC subsetsa
No Cytokine | IL-3 | GM-CSF | IFN-α | IFN-γ | TNF-α | TGF-β1 | IL-6 | |
---|---|---|---|---|---|---|---|---|
CD11c+ DCs | 36.5 ± 3.4 | 51.0 ± 3.3* | 79.5 ± 3.7** | 88.3 ± 2.8** | 83.8 ± 3.0** | 37.5 ± 2.8 | 34.5 ± 2.5 | 38.8 ± 3.5 |
CD11c− DCs | 19.5 ± 2.7 | 75.5 ± 3.3** | 45.3 ± 3.2* | 73.8 ± 3.1** | 30.0 ± 2.7* | 19.5 ± 2.3 | 18.8 ± 2.5 | 20.8 ± 2.5 |
a After 24-h culture with a panel of cytokines in the serum-free condition, the recovery of viable cells of each DC subset was counted using the trypan blue dye exclusion test. Freshly isolated cells were cultured in 96-well flat-bottom tissue culture plates at 4 × 104 cells in 200 μl of medium/well. Results are shown as percent recovery of viable cells (means ± SEM) of four separate experiments using DCs from healthy individual volunteers. Statistical significance using paired Student’s t test (∗, p < 0.05 and ∗∗, p < 0.001) is indicated.