Table III.

Organ distribution of CD4+ cells in RAG-2° mice reconstituted with CD4 subpopulations from IL-10° donorsa

Coinjected PopulationsPhenotype of Injected CD4+ SubpopulationsNo. of AnimalsNo. of Recovered CD4+ Cells (×104)b
SpleenLymph NodescBlooddIntestine
IL-10° CD25 CD45RBlow7138.0 ( ±49.6)24.5 (±6.3)27.4 (±9.8)79.6 (±36.2)
IL-10° CD25+ CD45RBlow778.0 (±23.5)8.8 ( ±2.5)8.1 (±2.2)1.5 (±0.3)
IL-10° CD45RBhigh7102.2 (±13.5)39.7 ( ±8.3)34.4 (±2.7)60.3 ( ±14.1)e
IL-10° CD25+IL-10° CD25+ CD45RBlow741.0 (±12.8)19.8 ( ±6.3)1.3 (±0.2)1.9 (±1.4)
+ wt CD45RBhighwt CD45RBhigh85.3 (±36.8)30.4 ( ±7.3)20.9 (±5.4)48.1 (±21.7)
  • a RAG-2° recipients were injected with 3 × 105 FACS-sorted CD4 T cells from IL-10° donors. In addition, 3 × 105 IL-10° CD25+ CD4 T cells were also injected with 3 × 105 wt CD45RBhigh CD4 T cells. Differentiation of the origin of the cells was achieved by using Ly5-congenic donors. The animals were analyzed for the presence of CD4+ cells in the indicated organs after 12–14 wk or when they dropped below 80% of their starting weight.

  • b Average ± SEM.

  • c Axillary, inguinal, and mesenteric lymph nodes.

  • d Data are expressed as cell number per milliliter of blood.

  • e n = 6; one animal, excluded from these data, contained more than 2000 × 104 CD4+ cells in the intestine. Including this animal results in an average CD4+ cell number of 352.1 (±292) × 104 in the intestine in this group.