Table II.

Organ distribution of CD4+ cells in RAG-2° mice reconstituted with coinjected CD4 subpopulationsa

Coinjected PopulationsNo. of AnimalsPhenotype of Injected CD4+ SubpopulationsNo. of Recovered CD4+ Cells (×104)b
SpleenLymph NodescBlooddIntestine
CD2510CD25139.0 ( ±68.1)28.2 (±7.7)16.0 (±4.0)26.0 (±10.8)
+ CD45BhighCD45RBhigh129.0 ( ±54.5)18.3 (±4.2)16.5 (±4.6)26.1 (±8.8)
CD25+6CD25+16.7 ( ±2.7)8.5 (±1.9)1.3 (±0.3)1.2 (±0.8)
+ CD45RBhighCD45RBhigh32.1 ( ±5.4)16.4 (±2.9)5.9 (±1.8)10.9 (±4.2)
  • a RAG-2° recipients were injected with 3 × 105 FACS-sorted CD4 T cells of either population as indicated. Differentiation of the origin of the cells was achieved by using Ly5-congenic donors. The animals were analyzed for the presence of CD4+ cells in the indicated organs after 12–14 wk or when they dropped below 80% of their starting weight.

  • b Average ± SEM.

  • c Axillary, inguinal, and mesenteric lymph nodes.

  • d Data are expressed as cell number per milliliter of blood.