Table II.

CD8+tg-TCR+ T cells proliferate in the spleen after transplantation of H2Kb+ heart but not H2Kb+ skin or islet allograftsa

DayAT OnlyB10 HeartB10 SkinB10 Islet
+11.5 ± 0.11.7 ± 0.2 NS1.4 ± 0.4 NS1.5 ± 0.8 NS
+35.8 ± 0.516.3 ± 2.9 p < 0.052.6 ± 0.2 NS5.2 ± 0.3 NS
+715.6 ± 4.444.8 ± 2.7 p < 0.0514.6 ± 4.2 NS19 ± 1.8 NS
  • a Purified CFSE-labeled CD8+ tg-TCR+ T cells (6 × 106) were adoptively transferred into immunodeficient mice. These mice then received either a heart skin or a islet allograft or were not transplanted the day after transfer. Spleen leukocytes were isolated from such mice 1, 3, and 7 days after transplantation and stained for expression of CD8 and the tg-TCR. This allowed analysis of the proliferation of the CD8+tg-TCR+ T cells as assessed by loss of CFSE fluorescence. Numbers indicate the percentage of CD8+tg-TCR+ cells that had divided at least once ± SD. Two to seven mice were used per group.