Table I.

Poly(I:C) induces CD11c+ DC, but not pre-DC2, to produce IFN-αβ and IL-12a

CellsIFN-α (pg/ml)IFN-β (pg/ml)IL-12 (pg/ml)
PBMC<12.5<8.3<7.7
Monocytes<12.5<8.3<7.7
CD11c+ DC24.156.628.5
Pre-DC2<12.5<8.3<7.7
Fibroblasts<12.528.6ND
  • a PBMC, monocytes, CD11c+ DC, pre-DC2 from the same donor(2 × 104/200 μl), or adult dermal fibroblasts (7.5 × 104/ml) were stimulated with 50 μg/ml poly(I:C) for 24 h, and cytokine concentrations in the supernatants were measured by ELISA. Data are representative of three experiments.