Table I.

Monokine production from monocytes stimulated with emetine-treated T cells + peptidea

MonokineT Cell Clones (HLA Restriction) for Stimulation (pg/ml)
BC20.7 (DR14)DT13.2 (DQ6)OT1.1 (DP5)
IL-1β105.024.021.0
IL-687.562.5140.0
IL-10172.5787.5725.0
IL-18<15.0<15.0<15.0
IL-12 (p40+ p70)145.075.020.5
GM-CSF475.01150.0887.5
TNF-α887.5642.5230.0
IL-10 / IL-1β1.632.834.5
  • a Emetine-treated T cells (BC20.7, DT13.2, and OT1.1) were cultured with peptide-prepulsed monocytes. The concentration of the peptides for each clonal response was 625-fold as much as the ED50 (5, 112.5, and 62.5 μM for BC20.7, DT13.2, and OT1.1, respectively). Culture supernatants after a 16-h (for IL-12), 24-h (for IL-1β, IL-10, IL-18, GM-CSF, and TNF-α), and 48-h (for IL-6) incubation were collected and subjected to ELISA. Results are expressed as the mean value of triplicate determinations. SE was <20%.