Table II.

Hierarchy of LLO- and p60-specific T cells in BALB/c and C57BL/6 mice

Mouse StrainPeptideSequenceTypeFrequencya (×10−5) ± SD
10–14 days p.i.6 wk p.i.
BALB/cLLO 91–99GYKDGNEYICD875.0 ± 8.347 ± 9.7
p60 217–225KYGVSVQDICD841.4 ± 11.825 ± 5.3
p60 476–484KYLVGFGRVCD818.1 ± 8.017 ± 5.1
LLO 189–200WNEKYAQAYPNVCD415.4 ± 8.210 ± 8.2
LLO 216–227QLIAKFGTAFKACD410.4 ± 1.77.1 ± 2.3
p60 449–457IYVGNGQMICD89.7 ± 4.26.5 ± 2.3
p60 367–378SSASAIIAEAQKCD47.6 ± 0.92.0 ± 1.0
LLO 211–222AYSESQLIAKFGCD45.4 ± 4.42.5 ± 1.6
p60 418–429QYASTTRISESQCD42.1 ± 0.70.4 ± 0.2
p60 300–311TEAAKPAPAPSTCD41.9 ± 0.40.6 ± 0.3
None0.6 ± 0.40.5 ± 0.2
C57BL/6LLO 190–201NEKYAQAYPNVSCD4125.0 ± 10.378.3 ± 13.3
LLO 318–329AFDAAVSGKSVSCD425.0 ± 3.016.7 ± 4.3
LLO 296–304VAYGRQVYLCD816.1 ± 5.39.2 ± 4.2
LLO 297–304AYGRQVYLCD812.7 ± 2.06.6 ± 2.6
LLO 253–264QIYYNVNVNEPTCD412.6 ± 1.05.1 ± 2.9
p60 401–412KYVFAKAGISLPCD45.1 ± 1.11.5 ± 0.5
p60 177–188TTQATTPAPKVACD44.9 ± 3.91.8 ± 1.0
None0.4 ± 0.20.6 ± 0.2
  • a The frequency of IFN-γ-secreting cells per 1 × 105 spleen cells is indicated as mean ± SD of three mice tested. ELISPOT assays were performed 10–14 days p.i. and 6 wk p.i. as indicated. Peptides are sorted according to the T cell frequency obtained when tested between day 10 and day 14 p.i. Bulk peptides with free acid at the carboxyl terminus were tested at a final concentration of 1 × 10−6 M.