Table III.

Flow cytometry analysis of PBL from donor A and B, cultured for 12 days with 10 μg/ml of SEI or SEKa

TCR VβbSEIcSEKc
Donor ADonor BDonor ADonor B
16.810.1
5.136.034.0
5.210.411.0
5.35.84.0
943.545.1
232.02.0
  • a Results are expressed as percentage of total cells in the culture.

  • b Results for TCR Vβ2, Vβ3, Vβ4, Vβ6.7, Vβ7, Vβ8, Vβ9, Vβ11, Vβ12, Vβ13.2, Vβ13.6, Vβ14, Vβ16, Vβ17, Vβ18, Vβ20, Vβ21.3, and Vβ22 were negatives.

  • c CD4/CD8 ratios were of 13.4 (donor A) and 21.7 (donor B) in SEI cultures (preferred expansion of the CD4 subset) and of 2.9 and 3.3 in SEK cultures (no gross alteration of CD4/CD8 in this case).