Table I.

Histamine up-regulates costimulatory molecule expression on DCa

Day 1Day 4
NoneHistamineTNF-αNoneHistamineTNF-α
CD40157 ± 16221 ± 23415 ± 52186 ± 21195 ± 25594 ± 69
CD49d11 ± 220 ± 428 ± 413 ± 214 ± 354 ± 9
CD54127 ± 21216 ± 32550 ± 61191 ± 29199 ± 34957 ± 84
CD8010 ± 119 ± 227 ± 512 ± 213 ± 247 ± 7
CD83b9 ± 210 ± 3114 ± 1910 ± 212 ± 4197 ± 23
(2% ± 1)(3% ± 1)(86% ± 11)(3% ± 1)(5% ± 1)(96% ± 4)
CD86b12 ± 4177 ± 21192 ± 3519 ± 525 ± 6253 ± 46
(5% ± 3)(65% ± 8)(81% ± 10)(8% ± 3)(11% ± 4)(95% ± 5)
HLA-DR334 ± 21555 ± 25968 ± 87447 ± 29489 ± 261695 ± 178
  • a Immature DC were either untreated or exposed to 10−5 M histamine or 20 ng/ml TNF-α. CD40, CD49d, CD54, CD80, CD83, CD86, and HLA-DR expression was analyzed by FACS after 1 and 4 days. Results are expressed in MFI values as mean ± SD of three separate experiments.

  • b In parentheses, the percentage of positive cells is shown as mean ± SD (n = 3).