Table III.

Inhibition of CTL induction by slow-sedimenting (3–4.5 mm/h) LPS-stimulated spleen cells in the presence of OX2:Fc depends upon their being MHC-matched with allo-stimulatory DC

LPS-Stimulateda Cells AddedOX2:Fc AddedPercent Lysis 51Cr Targets (50:1 E:T ratio)b
B10.BR anti-B10.Sgn (H2b/b)B10.Sgn anti-B10.BR (H2k/k)B10.BR anti-B10.D2 (H2d/d)
None40 ± 5.343 ± 5.552 ± 6.3
None+12 ± 3.2c13 ± 3.3c17 ± 4.4c
3–4.5 mm/h37 ± 6.442 ± 6.049 ± 5.9
3–4.5 mm/h+4.6 ± 2.2**12 ± 3.4c16 ± 4.2c
4.5–6 mm/h39 ± 6.340 ± 4.944 ± 5.6
4.5–6 mm/h+13 ± 3.4c13 ± 2.9c15 ± 4.0c
>6 mm/h17 ± 4.3c32 ± 4.4c28 ± 4.9c
>6 mm/h+7.2 ± 2.4c10 ± 2.3c13 ± 3.8c
  • a A total of 5 × 106 responder spleen cells were pooled from three 8-wk B10.BR (anti-B10, anti-B10.D2 response) or B10.Sgn (anti-B10.BR response) donors. Cells were cultured in triplicate with 2.5 × 106 mitomycin-c-treated allogeneic DC in the presence or absence of 1 × 106 cells from one of three pools of velocity-sedimented, LPS-stimulated, T-depleted, B10.Sgn spleen cells (Table I). Some cultures received additional OX2:Fc protein as described in Materials and Methods.

  • b Percent lysis (50:1, E:T) at 5 days using 1 × 104 51Cr spleen ConA targets.

  • c , p < 0.05, compared with control cultures in first row (ANOVA followed by pair-wise t test).

  • d ∗, p < 0.05, compared with all other groups (pair-wise t test).