Table II.

Cytokine production in 3C8 cultures stimulated with fMIGWIIa

StimulationIFN-γ (U/ml)TNF (pg/ml)IL-4 (pg/ml)IL-6 (pg/ml)IL-10 (pg/ml)
Unstimulated13 ± 3<100<80<160<500
fMIGWII1987 ± 358833± 361<801751± 864<500
  • a 3C8 cells (5 × 104) were cultured with 5 × 105 control or fMIGWII-pulsed irradiated APC in 200 μl in a round-bottom 96-well plate. Culture supernatant was collected after 48 h, and cytokines were measured as described in Materials and Methods.