Table II.

Total ECP content (micrograms per liter) does not increase in eosinophils cultured with GM-CSFa

Expt.Freshly PurifiedMedium (4 h)GM-CSF (4 h)Medium (8 h)GM-CSF (8 h)Medium (20 h)GM-CSF (20 h)
11623191017501962188619681816
22110216020002120204021201906
32410274028602800290028602760
  • a Eosinophils were cultured in the absence or presence of GM-CSF for the indicated times. Total ECP content was determined in Triton X-100-treated eosinophils (106/ml). Three independent experiments are shown.