Table III.

Cell lines inducing IFN-α production by PBMC in presence of SLE IgG

Cell LineaUV TreatmentIFN-α Production (U/ml)b
Normal IgGSLE IgGc
MonoMac62 ± 0102 ± 4
MonoMac6+2 ± 01058 ± 191
H99 ± 2292 ± 41
H9+2 ± 0276 ± 50
Jurkat2 ± 0109 ± 44
Jurkat+2 ± 0494 ± 64
U2662 ± 087 ± 5
U266+2 ± 0191 ± 89
None2 ± 013 ± 11
  • a Untreated or UV-treated (60 mJ) MonoMac6, H9, Jurkat, or U266 cells were first precultured for 4 h and then cultured for 24 h with IFN-α2b (500 U/ml)-costimulated normal PBMC. The cultures contained either normal IgG or SLE IgG (1 mg/ml).

  • b The figures represent IFN-α levels in culture medium (U/ml; means ± SD) measured by immunoassay.

  • c IgG derived from a 66-year-old male patient with a SLEDAI score of 14.