Table I.

Free release of proteinase 3 from activated PMN

ConditionaPercent Releaseb
PR3HLECG
Unstimulated PMN1.6 ± 0.5c1.6 ± 0.71.0 ± 0.4
LPS-primed and IL-8-stimulated PMN2.2 ± 0.52.2 ± 0.71.6 ± 0.4
PAF-primed and IL-8-stimulated PMN2.2 ± 0.52.2 ± 0.71.7 ± 0.5
TNF-α-primed and IL-8-stimulated PMN2.7 ± 1.12.5 ± 1.32.2 ± 1.4
  • a Cells were incubated at 37°C without agonists for 30 min, or primed for 5 min with 100 ng/ml LPS or 15 min with 10−9 M PAF or 100 U/ml TNF-α, then stimulated for 30 min with 10−8 M IL-8.

  • b Release of PR3, HLE, and CG was quantified in triplicate in cell-free supernatant fluids and cell extracts of unstimulated PMN using indirect binding ELISAs. The results are expressed as a percentage of the amount of each proteinase that was present in cell extracts from unstimulated cells.

  • c Data are mean values; error bars represent SD; n = 4 donors.