Table I.

Identification of vaccine-specific CD8+ T cells in PBMC of patients with metastatic melanoma by tHLA staining and IFN-γ expression

PatientTreatment209-2M tHLA+ (% total CD8+)IFN-γ
IC-FACSqRT-PCRa
None209b209-2Mc209d209-2Me209f209-2Mg
P1Pre0.060.060.040.080.0911
Post 20.040.070.070.030.0511
Post 40.040.020.020.000.0011
P2Pre2.462.222.390.030.0011
Post 30.430.260.470.000.0012
P3Pre0.060.080.080.000.0011
Post 20.300.100.090.140.1724
P4Pre0.080.090.080.010.0012
Post 20.070.100.090.030.0211
Post 40.270.180.200.050.10610
P5Pre0.000.000.000.000.0011
Post 20.050.030.050.000.0411
Post 40.600.140.040.220.3247
P6Pre0.020.040.040.020.0311
Post 40.170.100.070.080.1636
Post 80.630.250.200.110.051212
Post 120.550.370.230.220.2539
P7Pre0.080.030.010.000.0021
Post 40.080.070.080.000.0011
Post 80.860.070.060.070.007452
P8Pre0.110.000.120.200.3711
Post 42.350.170.361.001.3277155
  • a Increase in IFN-γ mRNA per 106 CD8 mRNA copies in stimulated vs unstimulated PBMC.

  • b tHLA-209 Spearman’s ρ = 0.746, p = 0.007.

  • c tHLA-209-2M Spearman’s ρ = 0.900, p = 0.001.

  • d IC-FACS 209 Spearman’s ρ = 0.785, p = 0.005.

  • e IC-FACS 209-2M Spearman’s ρ = 0.785, p = 0.005.

  • f qRT-PCR 209 Spearman’s ρ = 0.815, p = 0.003.

  • g q-RT-PCR 209-2M Spearman’s ρ = 0.900, p = 0.001.