Table I.

Checkerboard analysis of the effect of galectin-3 on the attraction of human peripheral blood monocytes in vitroa

BelowAbove
0 nM10 nM100 nM1000 nM
0 nM4.23 ± 0.757.55 ± 0.7910.7 ± 0.863.30 ± 2.82
10 nM8.66 ± 0.228.88 ± 1.0911.4 ± 2.113.25 ± 3.11
100 nM9.96 ± 0.729.23 ± 2.2310.5 ± 2.104.55 ± 3.69
1000 nM13.1 ± 1.3311.5 ± 3.4912.5 ± 2.873.50 ± 2.41
  • a Various concentrations of galectin-3 were applied to the lower chambers, and purified monocytes mixed with various concentrations of galectin-3 were applied to the upper chambers, as described in Materials and Methods. Monocyte migration is expressed as the percentage of migrated cells of the total cells. Data are the mean ± SD of four individual experiments.