Table II.

Stimulation of RF+ B cells by monoclonal anti-nucleosome Abs

AbIsotypeFine Specificity% Anti-IgM Proliferationa
RF+RF
PL 2-3IgG2ajH2A-H2B-DNA3176
PR 1-3IgG2ajH2A-H2B-DNA312−7
LG4-1IgG2ajNucleosome core particle1262
MRB4IgG2ajH2A-H2B-DNA13811
LG10-1IgG2ajH3-H4-DNA1549
LG8-1IgG2ajH2A-H2B-DNA489
PL 2-6IgG2bjH2A-H2B-DNA1−6
PL 9-7IgG3jH2A-H2B-DNA−6−16
PL 2-8IgG2bjH2A-H2B-DNA−1−1
PL 2-7IgG2bjH2A-H2B-DNA−40
MGC 23IgG2bjNucleosome core particle59
  • a CD40L-pretreated RF+ or RF B cells were cultured with 50 μg/ml designated mAbs, and proliferation was assessed at day 2.