Table I.

Quantitation of MHV-68 latency within subsets of spleen cells 14 days after infection

CellsMHV-68 Reciprocal Frequency (95% confidence limits)a% of CellsbTotal No. of CellscLatently Infected Cellsd
Expt. 1
Spleen1,521 (0–4,966)1002 × 1081.3 × 105
Mock sortinge7,596 (4,251–9,747)1002 × 1082.6 × 104
B cellsf12,555 (10,642–18,673)59.71.2 × 1089.5 × 103
Dendritic cellsg1,297 (377–1,971)6.71.3 × 1071 × 104
Macrophagesh3,571 (955–6,451)5.61.1 × 1073 × 103
Expt. 2
Spleen1,955 (239–3,731)1002 × 1081 × 105
Activated B cellsi890 (255–1,592)5.21 × 1071.1 × 104
Resting B cellsj35,208 (23,995–225,236)45.49.1 × 1072.5 × 103
  • a Frequency ± 95% confidence limits were determined by linear regression analysis of LDA data, as determined in Figs. 1 and 3.

  • b Percentage of each subset of total spleen cells was determined by FACS analysis.

  • c Total number of cells were estimated from the percentage of total spleen, based on an estimate of 2 × 108 cells/spleen at 14 days after infection.

  • d Number of latently infected cells based on the frequency of latency within each cell type and the estimated total number of cells.

  • e Mock sorted cells were stained and sorted for live, single events.

  • f B cells were sorted as B220+CD11bCD11c. The sorted populations were 99.24 ± 0.28% B cells, 0.15 ± 0.01% macrophages, and 0.73 ± 0.73% dendritic cells.

  • g Dendritic cells were sorted as B220CD11bCD11c+. The sorted populations were 95.60 ± 0.26% dendritic cells, 1.26 ± 0.55% macrophages, and 0.00 ± 0.00% B cells.

  • h Macrophages were sorted as B220CD11b+CD11c. The sorted populations were 95.59 ± 0.45% macrophages, 0.45 ± 0.35% dendritic cells, and 0.00 ± 0.00% B cells.

  • i Activated B cells were sorted as B220+PNAhigh.

  • j Resting B cells were B220+PNAlow.