Table I.

Percentage of tetramer-positive CD3+/CD8α+ T lymphocytes detected in fresh, unstimulated PBMC from DNA-vaccinated macaquesa

No. of DNA VaccinationsDays Post- DNA95045b95058b96031b961189612394004
0c0.020.040.010.010.000.02
414 0.21e 0.08 0.53
280.030.05
56 0.48 0.05 0.45
130.02 0.21 0.08 0.34
280.010.070.03d
  • a Tetramers typically stain <0.08% of fresh, unstimulated CD3+/CD8α+ T lymphocytes from PBL of naive Mamu-A*01+ animals.

  • b Thawed PBMC were used for samples from animals 95045, 95058, and 96031.

  • c Thawed PBMC from each animal were used to test levels of pre-DNA tetramer-positive cells.

  • d PBMC isolated 4 wk following animal 96031’s last (third, not fifth) DNA vaccination were tested.

  • e Significant responses >0.10% are in boldface.