Table II.

Phenotype analysis of immature DC after exposure to LPS and CD40L in presence of 1α,25-(OH)2D3a

Ctr + LPSD3 + LPSCtr + CD40LD3 + CD40L
Presentation molecules
MHCII369273396221
MHCI388221377297
Costimulatory/signaling molecules
CD8074488258
CD86NTNT10060
CD40290221369232
Maturation Ag
CD83298.73618
  • a Immature DC obtained after 7-day culture with GM-CSF and IL-4 were treated with 1α,25-(OH)2D3. After 72 h, cells were exposed to maturation-inducing stimuli LPS (10 ng/ml) or J558mCD40L for 48 h. Results are expressed as fluorescence intensity (FI). Data were expressed as mean (n = 4). NT, not tested.